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pcDNA3-ATR Kinase dead
(Plasmid #31612)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31612 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATR
  • Alt name
    ATM and Rad3 Related
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8000
  • Mutation
    K2327R (kinase dead)
  • Entrez Gene
    ATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Robert Abraham
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Plasmid contains an R1631H polymorphism. This is not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ATR Kinase dead was a gift from Aziz Sancar (Addgene plasmid # 31612 ; http://n2t.net/addgene:31612 ; RRID:Addgene_31612)
  • For your References section:

    Recruitment of DNA damage checkpoint proteins to damage in transcribed and nontranscribed sequences. Jiang G, Sancar A. Mol Cell Biol. 2006 Jan . 26(1):39-49. 10.1128/MCB.26.1.39-49.2006 PubMed 16354678