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pcDNA3.1(+) Flag-His-ATM kd
(Plasmid #31986)


Item Catalog # Description Quantity Price (USD)
Plasmid 31986 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days. See protocol -Transformation of the ATM Plasmid
  • Copy number


  • Gene/Insert name
    ATM kd
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    3'UTRf 9 nucl/D2870A N2875k. catalytically inactive.
  • GenBank ID
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI-SalI ligation (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Do not send out as a spot on paper, send out in liquid form.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) Flag-His-ATM kd was a gift from Michael Kastan (Addgene plasmid # 31986 ; ; RRID:Addgene_31986)
  • For your References section:

    Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD. Science. 1998 Sep 11;281(5383):1677-9. 10.1126/science.281.5383.1677 PubMed 9733515