Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3.1(+) Flag-His-ATM kd
(Plasmid #31986)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31986 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days. See protocol -Transformation of the ATM Plasmid
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATM kd
  • Alt name
    ATI
  • Alt name
    ATA
  • Alt name
    ATDC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9171
  • Mutation
    3'UTRf 9 nucl/D2870A N2875k. catalytically inactive.
  • GenBank ID
    NP_000042.3
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI-SalI ligation (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

Do not send out as a spot on paper, send out in liquid form.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) Flag-His-ATM kd was a gift from Michael Kastan (Addgene plasmid # 31986 ; http://n2t.net/addgene:31986 ; RRID:Addgene_31986)
  • For your References section:

    Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD. Science. 1998 Sep 11;281(5383):1677-9. 10.1126/science.281.5383.1677 PubMed 9733515