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BRCA1 (1-304)
(Plasmid #12645)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12645 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET151D topo
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E3-Ring domain, Ub-ligase
  • Alt name
    BRCA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    912
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site topo (unknown if destroyed)
  • 3′ cloning site topo (unknown if destroyed)
  • 5′ sequencing primer Universal T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BRCA1 (1-304) was a gift from Rachel Klevit (Addgene plasmid # 12645 ; http://n2t.net/addgene:12645 ; RRID:Addgene_12645)
  • For your References section:

    A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155