BRCA1 (1-304)
(Plasmid #12645)

• Depositing Lab: Rachel Klevit
• Publication: Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Backbone

• Vector backbone: pET151D topo
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 4500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E3-Ring domain, Ub-ligase
• Alt name: BRCA1
• Species: H. sapiens (human)
• Insert Size (bp): 912
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: topo (unknown if destroyed)
• 3′ cloning site: topo (unknown if destroyed)
• 5′ sequencing primer: Universal T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry