|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12647||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUbiquitin - wild type
Alt nameUbiquitin C
SpeciesH. sapiens (human)
Insert Size (bp)228
Entrez GeneUBC (a.k.a. HMG20)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
This plasmid contains a 76 amino acid synthetic ubiquitin insert used to determine the 3D structure of the UbcH5c/Ub noncovalent complex, as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ubiquitin WT was a gift from Rachel Klevit (Addgene plasmid # 12647 ; http://n2t.net/addgene:12647 ; RRID:Addgene_12647)
For your References section:A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155