Ube1/PET21d
(Plasmid #34965)

• Depositing Lab: Cynthia Wolberger
• Publication: Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2.

Backbone

• Vector backbone: PET21d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ube1
• Alt name: UBA1
• Species: H. sapiens (human)
• Insert Size (bp): 3176
• GenBank ID: NM_153280
• Entrez Gene: UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Articles Citing this Plasmid:
  • 27 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry