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pET15-UbE2D1
(Plasmid #15782)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15782 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15 modified
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin conjugating enzyme UbE2D1
  • Alt name
    UbE2D1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • GenBank ID
    NM_003338
  • Entrez Gene
    UBE2D1 (a.k.a. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • Xpress tag (enterokinase cleavage site) (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-UbE2D1 was a gift from Wade Harper (Addgene plasmid # 15782 ; http://n2t.net/addgene:15782 ; RRID:Addgene_15782)
  • For your References section:

    Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin J, Li X, Gygi SP, Harper JW. Nature. 2007 Jun 28. 447(7148):1135-8. 10.1038/nature05902 PubMed 17597759