pET3a-hUBA1
(Plasmid #63571)

• Purpose: Expression of human UBA1 tagged with His
• Depositing Lab: Titia Sixma
• Publication: Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20.

Backbone

• Vector backbone: pET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UBA1
• Alt name: Ubiquitin-activating enzyme E1
• Species: H. sapiens (human)
• Insert Size (bp): 3087
• Mutation: Isoform 2 (1018 amino acids)
• Entrez Gene: UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry