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A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination.
Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
Mol Cell. 2006 Mar 17. 21(6):873-80.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
12643UbcH5c WT pET28a
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12644UbcH5c S22R pET28a
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12645BRCA1 (1-304)
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12646BARD1 26-327 pET28a
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12647Ubiquitin WT
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12648Ubiquitin I44A
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