|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25696||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGary Nabel
- Backbone size w/o insert (bp) 7131
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)850
Entrez GeneSNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
The pPGS-CMV-CITE-neo vector allows the expression of chimeric transcripts encoding a gene of interest fused to the neomycin resistance gene. Expression of the neomycin resistance protein results from an internal ribosome entry site upstream of the neomycin open reading frame.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPGS-hSLUG.fl.flag was a gift from Eric Fearon (Addgene plasmid # 25696 ; http://n2t.net/addgene:25696 ; RRID:Addgene_25696)
Map uploaded by the depositor.