|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10905||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7032
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Alt nameSlug siRNA
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneSNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Sequence: 5'-CAG ACC CAT TCT GAT GTA AAG-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:siSlug3 was a gift from Bob Weinberg (Addgene plasmid # 10905 ; http://n2t.net/addgene:10905 ; RRID:Addgene_10905)
For your References section:The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta PB, Kuperwasser C, Brunet JP, Ramaswamy S, Kuo WL, Gray JW, Naber SP, Weinberg RA. Nat Genet. 2005 Oct . 37(10):1047-54. 10.1038/ng1634 PubMed 16142232