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siSlug1
(Plasmid #10903)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10903 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    siSlug
  • Alt name
    Slug siRNA
  • Alt name
    Slug
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    SNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Sequence: 5'-GAA CTG GAC ACA CAT ACA GTG-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    siSlug1 was a gift from Bob Weinberg (Addgene plasmid # 10903 ; http://n2t.net/addgene:10903 ; RRID:Addgene_10903)
  • For your References section:

    The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta PB, Kuperwasser C, Brunet JP, Ramaswamy S, Kuo WL, Gray JW, Naber SP, Weinberg RA. Nat Genet. 2005 Oct . 37(10):1047-54. 10.1038/ng1634 PubMed 16142232