|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31695||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5598
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)906
Entrez GeneSNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Contains Slug promoter (-908 to -2) sequence upstream of luciferase
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Slug_pGL2 was a gift from Paul Wade (Addgene plasmid # 31695 ; http://n2t.net/addgene:31695 ; RRID:Addgene_31695)
For your References section:MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA. Cell. 2003 Apr 18. 113(2):207-19. 10.1016/S0092-8674(03)00234-4 PubMed 12705869