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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 31694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL2_Basic
- Backbone size w/o insert (bp) 5598
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSnail
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Alt nameSNAI1
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SpeciesH. sapiens (human)
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Insert Size (bp)989
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GenBank IDNM_005985
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Entrez GeneSNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Contains Snail promoter (-1045 to -56) sequence upstream of luciferase
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Snail_pGL2 was a gift from Paul Wade (Addgene plasmid # 31694 ; http://n2t.net/addgene:31694 ; RRID:Addgene_31694) -
For your References section:
MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA. Cell. 2003 Apr 18. 113(2):207-19. 10.1016/S0092-8674(03)00234-4 PubMed 12705869