pINT-PuroDDHNF4a2
(Plasmid
#31443)
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Depositing Lab
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Depositing OrganizationUniversitaetsklinikum Essen
Located in Germany -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 31443-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 31443-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCSGFP
- Backbone size w/o insert (bp) 3500
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Vector typeMammalian Expression ; phiC31/attB
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHNF4A
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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Entrez GeneHNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
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Tag
/ Fusion Protein
- FKBP L106P (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Integration vector with attB site for phiC31 mediated integration of HNF4A2 into attP site using puromycin selection. HNF4A2 linked to destabilization domain (FKBP L106P) allowing shield inducibility.
DNA (Catalog # 31443-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 31443-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINT-PuroDDHNF4a2 was a gift from Gerhart Ryffel (Addgene plasmid # 31443 ; http://n2t.net/addgene:31443 ; RRID:Addgene_31443) -
For your References section:
Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483
Map uploaded by the depositor.
