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pCDNA3 5'cMyc-SMCl- S957A/S966A
(Plasmid #32372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32372 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32372-D50 50 μg of DNA in Tris buffer $495
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DNA 32372-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDNA3 cMyc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMC1A
  • Alt name
    SMCB
  • Alt name
    CDLS2
  • Alt name
    SMCILI
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • Mutation
    S957A/S996A
  • GenBank ID
    8243
  • Entrez Gene
    SMC1A (a.k.a. CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB)
  • Tag / Fusion Protein
    • c-Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECORl (not destroyed)
  • 3′ cloning site Xhol (not destroyed)
  • 5′ sequencing primer CMV-f
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 5'cMyc-SMCl- S957A/S966A was a gift from Michael Kastan (Addgene plasmid # 32372 ; http://n2t.net/addgene:32372 ; RRID:Addgene_32372)

  • For your References section:

    Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Kim ST, Xu B, Kastan MB. Genes Dev. 2002 Mar 1;16(5):560-70. 10.1101/gad.970602 PubMed 11877376
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