pCDNA3 -5'cMyc-SMCl- S957A
(Plasmid
#32368)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDNA3 cMyc
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSMC1A
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Alt nameSMCB
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Alt nameCDLS2
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Alt nameSMCILI
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SpeciesH. sapiens (human)
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Insert Size (bp)3700
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MutationS957A
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GenBank ID8243
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Entrez GeneSMC1A (a.k.a. CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB)
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Tag
/ Fusion Protein
- c-Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ECORl (not destroyed)
- 3′ cloning site Xhol (not destroyed)
- 5′ sequencing primer CMV-f
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 -5'cMyc-SMCl- S957A was a gift from Michael Kastan (Addgene plasmid # 32368 ; http://n2t.net/addgene:32368 ; RRID:Addgene_32368) -
For your References section:
Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Kim ST, Xu B, Kastan MB. Genes Dev. 2002 Mar 1;16(5):560-70. 10.1101/gad.970602 PubMed 11877376