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pcDNA mouse NHERF-1 (1-355)
(Plasmid #32705)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32705 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32705-D50 50 μg of DNA in Tris buffer $495
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DNA 32705-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
    NHERF-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    none
  • GenBank ID
    U74079
  • Entrez Gene
    Nherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
  • Promoter cmv
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site V= Hind3 i= XHho1 both filled in then blunted (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA mouse NHERF-1 (1-355) was a gift from Edward Weinman (Addgene plasmid # 32705 ; http://n2t.net/addgene:32705 ; RRID:Addgene_32705)

  • For your References section:

    Ezrin binding domain-deficient NHERF attenuates cAMP-mediated inhibition of Na(+)/H(+) exchange in OK cells. Weinman EJ, Steplock D, Wade JB, Shenolikar S. Am J Physiol Renal Physiol. 2001 Aug;281(2):F374-80. 10.1152/ajprenal.2001.281.2.F374 PubMed 11457730
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