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p3XFlag-CMV10-Flag-mMcl-1
(Plasmid #32978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32978 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32978-D50 50 μg of DNA in Tris buffer $495
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DNA 32978-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p3XFlag-CMV10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6307
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mcl-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    995
  • Entrez Gene
    Mcl1 (a.k.a. Gm52627, Mcl-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer acaaagaccatgacggtgat
  • 3′ sequencing primer ccaggagaggcactggggag
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFlag-CMV10-Flag-mMcl-1 was a gift from Joseph Opferman (Addgene plasmid # 32978 ; http://n2t.net/addgene:32978 ; RRID:Addgene_32978)

  • For your References section:

    Ubiquitin-independent degradation of antiapoptotic MCL-1. Stewart DP, Koss B, Bathina M, Perciavalle RM, Bisanz K, Opferman JT. Mol Cell Biol. 2010 Jun;30(12):3099-110. Epub 2010 Apr 12. 10.1128/MCB.01266-09 PubMed 20385764
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