|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35469||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5711
Modifications to backbone2 micron origin is from YEplac195, which has had its XbaI site removed.
Vector typeYeast 2 micron vector
- Promoter None
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Forward, T7
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSII326 was a gift from Steven Haase (Addgene plasmid # 35469 ; http://n2t.net/addgene:35469 ; RRID:Addgene_35469)
For your References section:New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222