Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pRSII326
(Plasmid #35469)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35469 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS32x
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 5711
  • Modifications to backbone
    2 micron origin is from YEplac195, which has had its XbaI site removed.
  • Vector type
    Yeast 2 micron vector
  • Promoter None
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Forward, T7
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel J. Lew, Duke University Medical Center
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSII326 was a gift from Steven Haase (Addgene plasmid # 35469 ; http://n2t.net/addgene:35469 ; RRID:Addgene_35469)
  • For your References section:

    New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222