pHHSDKXK (#19)
(Plasmid #35964)

• Depositing Lab: Thomas Sato
• Depositing Organization: Beth Israel Deaconess Medical Center
  Located in United States of America
• Publication: Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63.

Backbone

• Vector backbone: pBluescriptII SK (+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: This plasmid is not very stable (the bacteria lose or rearrange it quite often). Collect miniprep-DNA from freshly transfomed bacteria instead of midi- or maxipreps
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Tie2 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 2000

Gene/Insert 2

• Gene/Insert name: SDKLacZ

Gene/Insert 3

• Gene/Insert name: SV40 pA

Gene/Insert 4

• Gene/Insert name: Tie2 enhancer
• Species: M. musculus (mouse)
• Insert Size (bp): 1700

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Test

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Positive control DNA where LacZ is expressed under the control of Tie2 promoter and minimum enhancer fragment.

This plasmid is unstable in bacteria. The depositing laboratory recommends performing minipreps, as larger scale preps tend to result in recombination. To verify the plasmid, perform separate digests with KpnI and HindIII. KpnI should produce two bands at approximately 7kb and 3kb. HindIII should produce two bands at approximately 8kb and 2kb. See Addgene's diagnostic digest as an example.

DNA (Catalog # 35964-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 35964-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.