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CMV-Tol2 transposase
(Plasmid #158774)


Item Catalog # Description Quantity Price (USD)
Plasmid 158774 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2927
  • Total vector size (bp) 6108
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Tol2 tranposase
  • Species
  • Insert Size (bp)
  • Mutation
    Tol2 codon usage frequency well suitable for murine or human cells

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid obtained from Stefan Schulte-Merker.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.

Rapid BAC selection for tol2-mediated transgenesis in zebrafish. Bussman J, Schulte-Merker S. Development. 2011;138(19):4327-4332. doi:10.1242/dev.068080

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-Tol2 transposase was a gift from Hugo Snippert (Addgene plasmid # 158774 ; ; RRID:Addgene_158774)
  • For your References section:

    Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids. Heinz MC, Oost KC, Snippert HJG. STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18. 10.1016/j.xpro.2020.100126 PubMed 33377020