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Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
Heinz MC, Oost KC, Snippert HJG
STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
136257pLV 4xSTAR-mTFP-NLS-blastFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136258pLV 4xSTAR-mNeonGreen-NLS-blastFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136259pLV 4xSTAR-sTomato-NLS-blastFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136260pLV 4xSTAR-mScarletI-NLS-blastFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136261Tol2 8xSTAR-sTomato-NLS. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136262Tol2 8xSTAR-mNeonGreen-NLS. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136263Tol2 8xSTAR-mScarletI-NLS. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136264Tol2 8xSTAR-sTomato. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136266Tol2 8xSTAR-mScarletI. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
158774CMV-Tol2 transposaseExpression of Tol2 transposase

Antibodies from Article