Tol2 8xSTAR-sTomato. PGK-puro
(Plasmid
#136264)
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PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepminiTol2, Addgene #31829
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Backbone manufacturerStephen Ekker lab
- Backbone size w/o insert (bp) 3498
- Total vector size (bp) 6360
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Vector typeMammalian Expression ; Tol2 transposase
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namestem cell Ascl2 reporter, 8 repeats
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Alt name8x STAR
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)540
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Tag
/ Fusion Protein
- sTomato
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tol2 8xSTAR-sTomato. PGK-puro was a gift from Hugo Snippert (Addgene plasmid # 136264 ; http://n2t.net/addgene:136264 ; RRID:Addgene_136264) -
For your References section:
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids. Heinz MC, Oost KC, Snippert HJG. STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18. 10.1016/j.xpro.2020.100126 PubMed 33377020
Map uploaded by the depositor.
