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pRiboS EcoHind
(Plasmid #36254)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36254 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMV261
  • Backbone manufacturer
    Stover et al., 1991 Nature 351:456
  • Modifications to backbone
    The plasmid is derived from pMV261 (http://www.lablife.org/p?a=vdb_view&id=g2.7281169b992e40c865698d3455e3633c24b04b31). It contains a modified promoter.
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter Modified Hsp60

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The plasmid lacks the pAL5000 origin of replication and does not replicate in mycobacteria. This plasmid is related to pRiboS BsaHind, but is not reported in PMID 2227955333: Unlike pRiboS BsaHind, the original MCS is preserved, except that the BstBI site is lost.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRiboS EcoHind was a gift from Carolyn Bertozzi & Jessica Seeliger (Addgene plasmid # 36254 ; http://n2t.net/addgene:36254 ; RRID:Addgene_36254)
  • For your References section:

    A riboswitch-based inducible gene expression system for mycobacteria. Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. 10.1371/journal.pone.0029266 PubMed 22279533