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pST5552
(Plasmid #36255)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36255 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMV261
  • Backbone manufacturer
    Stover et al., 1991 Nature 351:456
  • Total vector size (bp) 4357
  • Modifications to backbone
    The plasmid is derived from pMV261 (http://www.lablife.org/p?a=vdb_view&id=g2.7281169b992e40c865698d3455e3633c24b04b31). It contains a modified promoter and MCS (see reference).
  • Vector type
    Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717
  • Promoter ModifiedHsp60

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer rrnB-T1-term-Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The plasmid is high copy in E. coli, but low copy in mycobacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST5552 was a gift from Carolyn Bertozzi & Jessica Seeliger (Addgene plasmid # 36255 ; http://n2t.net/addgene:36255 ; RRID:Addgene_36255)
  • For your References section:

    A riboswitch-based inducible gene expression system for mycobacteria. Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. 10.1371/journal.pone.0029266 PubMed 22279533