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pST5832
(Plasmid #36256)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36256 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMV261
  • Backbone manufacturer
    Stover et al., 1991 Nature 351:456
  • Total vector size (bp) 4357
  • Modifications to backbone
    The plasmid is derived from pMV261 (http://www.lablife.org/p?a=vdb_view&id=g2.7281169b992e40c865698d3455e3633c24b04b31). It contains a modified promoter and MCS (see reference).
  • Vector type
    Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacZ
  • Alt name
    beta-D-galactosidase
  • Alt name
    bgal
  • Species
    E. coli
  • Insert Size (bp)
    3075

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13_for20
  • 3′ sequencing primer rrnB-T1-term-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The plasmid is high copy in E. coli, but low copy in mycobacteria.

Cloning was done via assembly PCR, sot he XbaI-HindIII fragment comprises the promoter-riboswitch. The insert is 3361bp including the promoter. There are mismatches between Addgene's quality control sequence and the depositor sequence, but they are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST5832 was a gift from Carolyn Bertozzi & Jessica Seeliger (Addgene plasmid # 36256 ; http://n2t.net/addgene:36256 ; RRID:Addgene_36256)
  • For your References section:

    A riboswitch-based inducible gene expression system for mycobacteria. Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. 10.1371/journal.pone.0029266 PubMed 22279533