UAS::PTPRFa.1-DN-GFP
(Plasmid #37110)

• Depositing Lab: Alvaro Sagasti
• Depositing Organization: University of California, Los Angeles (UCLA)
  Located in United States of America
• Publication: Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub 2012 Feb 9.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone manufacturer: Chi-Bien Chien
• Backbone size w/o insert (bp): 5883
• Total vector size (bp): 10018
• Modifications to backbone: ccdB gene eliminated during Gateway recombination
• Vector type: Tol2 Gateway Expression Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UAS::PTPRFa.1-DN-GFP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 5500
• Mutation: deleted amino acids 1326-1894
• GenBank ID: JQ414007
• Entrez Gene: ptprf (a.k.a. LAR, zf-LAR)
• Promoter: UAS-E1b
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PTPRFa sequence ends at aa1326 and is followed by the short sequence NPAFLYKVAT immediately before EGFP.

DNA (Catalog # 37110-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 37110-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.