TagRFP-T-Rabenosyn-5
(Plasmid #37537)

• Depositing Lab: Silvia Corvera
• Depositing Organization: University of Massachusetts, Worcester
  Located in United States of America
• Publication: Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 30.
• Citations: 2 Articles

Backbone

• Vector backbone: GFP-Rabenosyn5
• Backbone manufacturer: Corvera Lab
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7044
• Modifications to backbone: TagRFP-T from Roger Tsien was used to replace the GFP in the Corvera Lab GFP-Rabenosyn5.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rabenosyn5
• Species: H. sapiens (human)
• Insert Size (bp): 2354
• GenBank ID: 40034008
• Entrez Gene: RBSN (a.k.a. Rabenosyn-5, ZFYVE20)
• Promoter: CMV
• Tag / Fusion Protein:
  • TagRFP-T (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Designed and prepared by Deborah Leonard, Deanna Navaroli and Anil Chawla (Corvera Lab)

There are a few sequence discrepancies between the full sequence and the Addgene QC sequence--these do not affect the function of the plasmid, but the XhoI site located at the 3' end of the TagRFP-T on the depositor's map is NOT present in the vector.

DNA (Catalog # 37537-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 37537-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.