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TagRFP-T-EEA1
(Plasmid #42635)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42635 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4236
  • Modifications to backbone
    TagRFP-T from Roger Tsien was used to replace the GFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EEA1
  • Alt name
    Early Endosome Antigen 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4236
  • Mutation
    none
  • GenBank ID
    NM_003566
  • Promoter CMV
  • Tag / Fusion Protein
    • TagRFP-T (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (destroyed during cloning)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TagRFP-T-EEA1 was a gift from Silvia Corvera (Addgene plasmid # 42635)
  • For your References section:

    Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Navaroli DM, Bellve KD, Standley C, Lifshitz LM, Cardia J, Lambright D, Leonard D, Fogarty KE, Corvera S. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 30. 10.1073/pnas.1115495109 PubMed 22308388