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TagRFP-T-EEA1
(Plasmid #42635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42635 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 42635-D50 50 μg of DNA in Tris buffer $495
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DNA 42635-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4236
  • Modifications to backbone
    TagRFP-T from Roger Tsien was used to replace the GFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EEA1
  • Alt name
    Early Endosome Antigen 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4236
  • Mutation
    none
  • GenBank ID
    NM_003566
  • Entrez Gene
    EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
  • Promoter CMV
  • Tag / Fusion Protein
    • TagRFP-T (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (destroyed during cloning)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TagRFP-T-EEA1 was a gift from Silvia Corvera (Addgene plasmid # 42635 ; http://n2t.net/addgene:42635 ; RRID:Addgene_42635)

  • For your References section:

    Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Navaroli DM, Bellve KD, Standley C, Lifshitz LM, Cardia J, Lambright D, Leonard D, Fogarty KE, Corvera S. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 30. 10.1073/pnas.1115495109 PubMed 22308388
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