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pcDNA3-ShhN-Ren
(Plasmid #37677)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37677 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 37677-D50 50 μg of DNA in Tris buffer $495
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DNA 37677-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shh
  • Species
    M. musculus (mouse)
  • Mutation
    soluble form (see comments)
  • Entrez Gene
    Shh (a.k.a. 9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, ShhNC)
  • Promoter CMV
  • Tag / Fusion Protein
    • renilla luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.

Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ShhN-Ren was a gift from Philip Beachy (Addgene plasmid # 37677 ; http://n2t.net/addgene:37677 ; RRID:Addgene_37677)

  • For your References section:

    Hedgehog-mediated patterning of the mammalian embryo requires transporter-like function of dispatched. Ma Y, Erkner A, Gong R, Yao S, Taipale J, Basler K, Beachy PA. Cell. 2002 Oct 4;111(1):63-75. 10.1016/S0092-8674(02)00977-7 PubMed 12372301
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