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PJ-Sac
(Plasmid #38000)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38000 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmRFP-C1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8010
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FKBP1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    318
  • GenBank ID
    NM_054014
  • Entrez Gene
    FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    SAC1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1545
  • GenBank ID
    NM_001179777

Gene/Insert 3

  • Gene/Insert name
    INPP5E
  • Species
    H. sapiens (human)
  • Mutation
    Asp 1263 mutated to Ala
  • GenBank ID
    NM_019892

Resource Information

Depositor Comments

Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PJ-Sac was a gift from Robin Irvine (Addgene plasmid # 38000 ; http://n2t.net/addgene:38000 ; RRID:Addgene_38000)
  • For your References section:

    PI4P and PI(4,5)P2 Are Essential But Independent Lipid Determinants of Membrane Identity. Hammond GR, Fischer MJ, Anderson KE, Holdich J, Koteci A, Balla T, Irvine RF. Science. 2012 Jun 21. 10.1126/science.1222483 PubMed 22722250