PJ-DEAD
(Plasmid #38002)

• Depositing Lab: Robin Irvine
• Depositing Organization: University of Cambridge
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Hammond et al Science. 2012 Jun 21.
• Citations: 10 Articles

Backbone

• Vector backbone: pmRFP-C1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 8010
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: FKBP1A
• Species: H. sapiens (human)
• Insert Size (bp): 318
• GenBank ID: NM_054014
• Entrez Gene: FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: SAC1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1545
• Mutation: Cys 779 mutated to Ser
• GenBank ID: NM_001179777
• Entrez Gene: SAC1 (a.k.a. YKL212W, RSD1)

Gene/Insert 3

• Gene/Insert name: INPP5E
• Species: H. sapiens (human)
• Mutation: Asp 1263 mutated to Ala
• GenBank ID: NM_019892
• Entrez Gene: INPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tamas Balla

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.

DNA (Catalog # 38002-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 38002-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.