Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #38011)


Item Catalog # Description Quantity Price (USD)
Plasmid 38011 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5340
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Clathrin, light polypeptide (LCa)
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Clta (a.k.a. Lca)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EYFP (C terminal on backbone)
    • SNAP26m (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clathrin light chain was a kind of gift of Dr. James H. Keen (Kimmel Cancer Center).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSNAP-CLC-EYFP was a gift from Xiaowei Zhuang (Addgene plasmid # 38011 ; ; RRID:Addgene_38011)
  • For your References section:

    Fast, three-dimensional super-resolution imaging of live cells. Jones SA, Shim SH, He J, Zhuang X. Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8. 10.1038/nmeth.1605 PubMed 21552254