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GFP-CHC17KDP
(Plasmid #59799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59799 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4697
  • Total vector size (bp) 10646
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    On transformation of E. coli with normal protocols, colonies take longer to form and are very small. If you have trouble growing the bugs, pick a few colonies and grow at 37°C, 220rpm in 5ml aliquots of LB + Kanamycin (half-strength). If there is little growth after 18-24 h, re-seed 0.5 ml of the culture into a fresh 5 ml aliquot of LB + kanamycin (half-strength). Grow at 37 °C, 220 rpm. Repeat if necessary. Larger volumes can be obtained by normal protocols.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHC17
  • Alt name
    CLTC
  • Alt name
    CHC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5949
  • Mutation
    Silent mutations in amino acids 61-65 to confer siRNA resistance.
  • GenBank ID
    NM_001288653.1
  • Entrez Gene
    CLTC (a.k.a. CHC, CHC17, CLH-17, CLTCL2, Hc, MRD56)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer pEGFP-C2_FP
  • 3′ sequencing primer pEGFP-C2_RP
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-CHC17KDP was a gift from Stephen Royle (Addgene plasmid # 59799 ; http://n2t.net/addgene:59799 ; RRID:Addgene_59799)
  • For your References section:

    A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. Booth DG, Hood FE, Prior IA, Royle SJ. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. 10.1038/emboj.2011.15 PubMed 21297582