GFP-CHC17KDP
(Plasmid #59799)

• Purpose: Expresses knockdown-proof human CHC17 tagged with GFP.
• Depositing Lab: Stephen Royle
• Publication: Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4697
• Total vector size (bp): 10646
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: On transformation of E. coli with normal protocols, colonies take longer to form and are very small. If you have trouble growing the bugs, pick a few colonies and grow at 37°C, 220rpm in 5ml aliquots of LB + Kanamycin (half-strength). If there is little growth after 18-24 h, re-seed 0.5 ml of the culture into a fresh 5 ml aliquot of LB + kanamycin (half-strength). Grow at 37 °C, 220 rpm. Repeat if necessary. Larger volumes can be obtained by normal protocols.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHC17
• Alt name: CLTC
• Alt name: CHC
• Species: H. sapiens (human)
• Insert Size (bp): 5949
• Mutation: Silent mutations in amino acids 61-65 to confer siRNA resistance.
• GenBank ID: NM_001288653.1
• Entrez Gene: CLTC (a.k.a. CHC, CHC17, CLH-17, CLTCL2, Hc, MRD56)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: pEGFP-C2_FP
• 3′ sequencing primer: pEGFP-C2_RP

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry