PurposeLocalization: Clathrin Vesicles, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54040||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCLTB (a.k.a. LCB)
- Promoter CMV
/ Fusion Protein
- mEmerald (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Clathrin Vesicles
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Clathrin-15 was a gift from Michael Davidson (Addgene plasmid # 54040 ; http://n2t.net/addgene:54040 ; RRID:Addgene_54040)
For your References section:Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 10.1073/pnas.1119262109. Epub 2012 Mar 19. 10.1073/pnas.1119262109 PubMed 22431626