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GFP-FKBP-LCa
(Plasmid #59353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59353 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 59353-D50 50 μg of DNA in Tris buffer $495
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DNA 59353-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4676
  • Total vector size (bp) 5781
  • Modifications to backbone
    Insertion of FKBP domain to tag gene of interest at N-terminus.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Clathrin Light Chain A
  • Alt name
    LCa
  • Alt name
    CLTA
  • Alt name
    non-neuronal isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    657
  • GenBank ID
    NM_001833.3
  • Entrez Gene
    CLTA (a.k.a. LCA)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • FKBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site Acc65I (unknown if destroyed)
  • 5′ sequencing primer pEGFP-C2_FP
  • 3′ sequencing primer pEGFP-C2_RP
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    LCa: IMAGE 3944942

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-FKBP-LCa was a gift from Stephen Royle (Addgene plasmid # 59353 ; http://n2t.net/addgene:59353 ; RRID:Addgene_59353)

  • For your References section:

    Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. 10.1242/jcs.124834 PubMed 23532825
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