Abr-pLEF
(Plasmid #38157)

• Depositing Lab: Nora Heisterkamp
• Publication: Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30. Epub 2007 Dec 10.

Backbone

• Vector backbone: pLEF
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 10200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABR
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• GenBank ID: NM_001092
• Entrez Gene: ABR (a.k.a. MDB)
• Promoter: SV40
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GST or T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences.

Addgene sequencing found an E24G point mutation--this does not alter plasmid function.