pCI-neo.ARRDC1-mCherry
(Plasmid #38319)

• Depositing Lab: Quan Lu
• Publication: Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51. Epub 2012 Feb 6.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 7460
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARRDC1
• Species: H. sapiens (human)
• Insert Size (bp): 2010
• GenBank ID: NM_152285.2
• Entrez Gene: ARRDC1 (a.k.a. RP11-48C7.5)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T3 promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry