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pEF6.mCherry-TSG101
(Plasmid #38318)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38318 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5780
  • Total vector size (bp) 7666
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSG101
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1881
  • GenBank ID
    NM_006292.3
  • Entrez Gene
    TSG101 (a.k.a. TSG10, VPS23)
  • Promoter EF1
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that there is a mutation in the TSG101 ORF, Y82H. This mutation should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6.mCherry-TSG101 was a gift from Quan Lu (Addgene plasmid # 38318 ; http://n2t.net/addgene:38318 ; RRID:Addgene_38318)
  • For your References section:

    Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51. Epub 2012 Feb 6. 10.1073/pnas.1200448109 PubMed 22315426