|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62964||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepEGFP C2
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCD63 molecule
SpeciesH. sapiens (human)
Insert Size (bp)749
MutationCD63 has 1 silent mutation compared to the coding sequence of NM_001267698.1 N129 AAT instead of AAC
Entrez GeneCD63 (a.k.a. LAMP-3, ME491, MLA1, OMA81H, TSPAN30)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer 5’ CCGACAACCACTACCTGAGC 3’
- 3′ sequencing primer 5’ ACAAACCACAACTAGAATGCAG 3’ (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Examples of use:
Blagoveshchenskaya AD, Hannah MJ, Allen S, Cutler DF. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor. Mol Biol Cell 13:1582-1593.
Bampton ET, Goemans CG, Niranjan D, Mizushima N, Tolkovsky AM. (2005) The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
Autophagy 1: 23-36.
Harrison-Lavoie KJ, Michaux G, Hewlett L, Kaur J, Hannah MJ, Lui-Roberts WW, Norman KE, Cutler DF. (2006) P-selectin and CD63 use different mechanisms for delivery to Weibel-Palade bodies.
Traffic 7: 647-662.
Babich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T. (2008) Selective release of molecules from Weibel-Palade bodies during a lingering kiss. Blood 111: 5282-5290.
Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD63-pEGFP C2 was a gift from Paul Luzio (Addgene plasmid # 62964 ; http://n2t.net/addgene:62964 ; RRID:Addgene_62964)