phOct4-EGFP
(Plasmid #38776)

• Depositing Lab: Wei Cui
• Depositing Organization: University of Edinburgh, Roslin Institute
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Gerrard et al Stem Cells. 2005;23(1):124-33.
• Citations: 4 Articles

Backbone

• Vector backbone: pEGFP-1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Oct-4 promoter
• Species: H. sapiens (human)
• Insert Size (bp): 3950
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Promoter: human OCT4
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: TT CCC ATG TCA AGT AAG TGG GGT GG
• 3′ sequencing primer: CGA GAA GGC AAA ATC TGA AGC CAG G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human OCT4 promoter (hOCT4pr, from 67539 to 71490 in human DNA sequence with accession number AP000509) was amplified by PCR with primers: hOCT4pr-F (5'−TTCCCATGTCAAGTAAGTGGGGTGG-3') and hOCT4pr-R (5'-CGAGAAGGCAAAATCTGAAGCCAGG-3') using human genomic DNA (Promega G3041) as a template. The fragment was cloned into a TOPO vector and the hOCT4 promoter was subsequently cloned into vector pEGFP1 by insertion into HindIII and AgeI restriction enzyme sites upstream of EGFP.

DNA (Catalog # 38776-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 38776-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.