|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21321||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonePL-SIN lentiviral vector
Backbone manufacturerBuzina et al., PLoS Genetics, 2008
- Backbone size w/o insert (bp) 6531
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameEnhanced Green Fluorescence Protein
Insert Size (bp)720
- Promoter Nanog promoter region
- Cloning method Unknown
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
EGFP driven by Nanog promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PL-SIN-Nanog-EGFP was a gift from James Ellis (Addgene plasmid # 21321 ; http://n2t.net/addgene:21321 ; RRID:Addgene_21321)
For your References section:Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J. Nat Methods. 2009 May . 6(5):370-6. 10.1038/nmeth.1325 PubMed 19404254