Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency.
Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
Nat Methods. 2009 May . 6(5):370-6. PubMed Article
Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
Nat Methods. 2009 May . 6(5):370-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 21312 | PL-SIN-PGK-EiP | Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance |
| 21313 | PL-SIN-EOS-C(3+)-EiP | Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance |
| 21314 | PL-SIN-EOS-S(4+)-EiP | Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance |
| 21315 | pMXs-mRFP1 | MoMLV-based Retroviral vector, expresses mRFP1 |
| 21316 | PL-SIN-PGK-EGFP | Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP |
| 21317 | PL-SIN-EOS-S(4+)-EGFP | Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP |
| 21318 | PL-SIN-EOS-C(3+)-EGFP | Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP |
| 21319 | PL-SIN-Oct4-EGFP | Lentiviral vector, expresses EGFP under Oct3/4 promoter |
| 21320 | PL-SIN-EF1α-EGFP | Control Lentiviral vector with strong EF1a promoter, expresses EGFP |
| 21321 | PL-SIN-Nanog-EGFP | Lentiviral vector, expresses EGFP under Nanog promoter |