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pBA2kIKmA2
(Plasmid #39213)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39213 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 39213-D50 50 μg of DNA in Tris buffer $495
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DNA 39213-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Synthetic Biology
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kudzu Isoprene Synthase
  • Alt name
    kIspS
  • Species
    Synthetic; Pueraria montana
  • Insert Size (bp)
    1719
  • Mutation
    Deleted the predicted chloroplast transit peptide
  • GenBank ID
    AY316691
  • Promoter psbA2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI, NcoI (not destroyed)
  • 3′ cloning site XbaI, BamHI (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bentley FK, Melis A (2012) Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms. Biotech Bioeng 109:100-109

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA2kIKmA2 was a gift from Anastasios Melis (Addgene plasmid # 39213 ; http://n2t.net/addgene:39213 ; RRID:Addgene_39213)

  • For your References section:

    Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Lindberg P, Park S, Melis A. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13. 10.1016/j.ymben.2009.10.001 PubMed 19833224
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