Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #53262)


Item Catalog # Description Quantity Price (USD)
Plasmid 53262 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England BioLabs
  • Backbone size w/o insert (bp) 4245
  • Total vector size (bp) 13581
  • Modifications to backbone
    A 9.34 kB BglII-BglII genomic fragment, containing a seven gene carotenoid pathway cluster from Erwinia herbicola (Pantoea agglomerans) Eho10, was excised from plasmid pPL376, and inserted into the BamHI site of pACYC184 in the forward orientation.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best carotenoid production, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    Low Copy


  • Gene/Insert name
    crtE, idi, crtX, crtY, crtI, crtB, crtZ
  • Alt name
    the idi gene was earlier referred to as ORF6
  • Species
    Erwinia herbicola Eho10
  • Insert Size (bp)
  • GenBank ID
  • Promoter endogenous promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The E. herbicola carotenoid gene cluster was excised from plasmid pPL376, obtained from the late Robert W. Tuveson. See: 1. Tuveson, R.W., Larson, R.A., and Kagan, J. (1986) Role of cloned carotenoid genes expressed in Escherichia coli in protecting against inactivation by near-UV light and specific phototoxic molecules. J. Bacteriol. 170, 4675-4680. 2. Perry, K.L., Simonitch, T.A., Harrison-Lavoie, K. J., and Liu, S. (1986) Cloning and regulation of frwinia herbicola pigment genes. J. Bacteriol. 168, 607-612. 3. Hundle,B., Alberti,M., Nievelstein,V., Beyer,P., Kleinig,H., Armstrong,G.A., Burke,D.H. and Hearst,J.E.(1994) Functional assignment of Erwinia herbicola Eho10 carotenoid genes expressed in Escherichia coli Mol. Gen. Genet. 245, 406-416.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-EHER was a gift from Francis X Cunningham Jr (Addgene plasmid # 53262 ; ; RRID:Addgene_53262)
  • For your References section:

    Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E. Plant Cell. 1994 Aug;6(8):1107-21. 10.1105/tpc.6.8.1107 PubMed 7919981