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(Plasmid #53287)


Item Catalog # Description Quantity Price (USD)
Plasmid 53287 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Francis X. Cunningham, Jr.
  • Backbone size w/o insert (bp) 13581
  • Total vector size (bp) 10040
  • Modifications to backbone
    pAC-EHER was digested with DrdI and partially digested with BsaI, and a resulting 10.64 kB fragment was gel-purified, blunted with the Klenow enzyme, and ligated to give pAC-EHER10.6 (unpublished). This latter plasmid was then digested with SphI, partially digested with DraIII, the ends were made blunt with the Klenow enzyme, and a gel-purified 10.04 kB fragment was then ligated to give pAC-ZEAXipi.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Grow liquid cultures on a platform shaker at 28 degrees Celsius in darkness for 2-3 days for best zeaxanthin production, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    Low Copy


  • Gene/Insert name
    crtE, idi, crtY, crtI, crtB, crtZ
  • Alt name
    idi also known as ORF6
  • Species
    Erwinia herbicola Eho10
  • GenBank ID
  • Promoter endogenous promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (destroyed during cloning)
  • 3′ cloning site DraIII (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-ZEAXipi was a gift from Francis X Cunningham Jr (Addgene plasmid # 53287 ; ; RRID:Addgene_53287)
  • For your References section:

    A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Cunningham FX Jr, Gantt E. Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. 10.1007/s11120-007-9210-0 PubMed 17634749