Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #39228)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 39228 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HERC2 HECT Domain
  • Alt name
  • Alt name
    HECT and RLD domain containing E3 ubiquitin protein ligase 2
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
    NM_004667.5 Uniprot: O95714[4457-4794]
  • Entrez Gene
    HERC2 (a.k.a. D15F37S1, MRT38, SHEP1, jdf2, p528)
  • Promoter T7
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HECT domain active site point mutation (C4762A substitution)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.Flag.HERC2-HECT-C4762A.6xHis was a gift from Aziz Sancar (Addgene plasmid # 39228 ; ; RRID:Addgene_39228)
  • For your References section:

    Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein. Kang TH, Reardon JT, Sancar A. Nucleic Acids Res. 2011 Apr;39(8):3176-87. Epub 2010 Dec 30. 10.1093/nar/gkq1318 PubMed 21193487