|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39526||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid #1764
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameHRAS V12
SpeciesH. sapiens (human)
MutationV12 = G12V
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE_5 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABEpuro-H-RAS_V12 was a gift from Julian Downward (Addgene plasmid # 39526 ; http://n2t.net/addgene:39526 ; RRID:Addgene_39526)