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pCDH-puro-cMyc
(Plasmid #46970)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46970 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PCDH-CMV-MCS-EF1-PURO
  • Backbone manufacturer
    System Biosciences
  • Total vector size (bp) 7384
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MYC
  • Alt name
    v-myc avian myelocytomatosis viral oncogene homolog
  • Alt name
    c-MYC
  • Alt name
    MRTL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1323
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-puro-cMyc was a gift from Jialiang Wang (Addgene plasmid # 46970 ; http://n2t.net/addgene:46970 ; RRID:Addgene_46970)
  • For your References section:

    Inhibition of BET bromodomain targets genetically diverse glioblastoma. Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S, Knapp S, Wang J. Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12. 10.1158/1078-0432.CCR-12-3066 PubMed 23403638