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Lenti-sh1368 knockdown c-myc
(Plasmid #29435)


Item Catalog # Description Quantity Price (USD)
Plasmid 29435 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    FUGW, modified
  • Backbone manufacturer
    David Baltimore (Caltech) and Valerie
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
    c-Myc shRNA
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • WPRE (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments


The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008).

To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation.

See the attached document for a map from Lien et al., 2008 for vector information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-sh1368 knockdown c-myc was a gift from Bob Eisenman (Addgene plasmid # 29435 ; ; RRID:Addgene_29435)
  • For your References section:

    Myc-regulated microRNAs attenuate embryonic stem cell differentiation. Lin CH, Jackson AL, Guo J, Linsley PS, Eisenman RN. EMBO J. 2009 Oct 21. 28(20):3157-70. 10.1038/emboj.2009.254 PubMed 19745813