Lenti-sh1368 knockdown c-myc
(Plasmid #29435)

• Depositing Lab: Bob Eisenman
• Publication: Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Backbone

• Vector backbone: FUGW, modified
• Backbone manufacturer: David Baltimore (Caltech) and Valerie
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-Myc shRNA
• Alt name: c-Myc
• Species: H. sapiens (human)
• Insert Size (bp): 60
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • WPRE (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Supplemental Documents:
  • FUGW-H1 (modified)
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

sh1368 sequence= GACGAGAACAGTTGAAACA

The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008).

To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation.

See the attached document for a map from Lien et al., 2008 for vector information.